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验证数据展示

  • WB analysis using 12238-1-AP (same clone as 12238-1-PBS)

    WB analysis using 12238-1-AP (same clone as 12238-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

    IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

    IHC staining of human prostate cancer using 12238-1-AP (same clone as 12238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

    IF Staining of U2OS using 12238-1-AP (same clone as 12238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12238-1-AP (same clone as 12238-1-PBS)

    IP experiment of HeLa using 12238-1-AP (same clone as 12238-1-PBS)

    IP result of anti-MKRN2 (IP:12238-1-AP, 4ug; Detection:12238-1-AP 1:2000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 12238-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MKRN2 Polyclonal antibody, PBS Only

MKRN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12238-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

E3 ubiquitin-protein ligase makorin-2, EC:2.3.2.27, HSPC070, makorin ring finger protein 2, RING finger protein 62

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12238-1-PBS targets MKRN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2881

Product name: Recombinant human MKRN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 67-416 aa of BC015715

Sequence: GTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 makorin ring finger protein 2
别名 E3 ubiquitin-protein ligase makorin-2, EC:2.3.2.27, HSPC070, makorin ring finger protein 2, RING finger protein 62
计算分子量 416 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC015715
基因名称 MKRN2
Gene ID (NCBI) 23609
RRIDAB_2142636
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H000
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.

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