验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
12238-1-AP targets MKRN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | MKRN2 fusion protein Ag2881 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | makorin ring finger protein 2 |
| 别名 | RING-type E3 ubiquitin transferase makorin-2, RING finger protein 62, makorin ring finger protein 2, HSPC070, EC:2.3.2.27 |
| 计算分子量 | 416 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC015715 |
| 基因名称 | MKRN2 |
| Gene ID (NCBI) | 23609 |
| RRID | AB_2142636 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H000 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| IHC protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| IF protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation | ||
Biochem Biophys Res Commun Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.
| ||
Oncol Lett Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation.
| ||
J Invest Dermatol N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing | ||
Cancer Biomark MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53 |
