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验证数据展示

  • ChIP experiment of K-562 using 83803-2-RR

    ChIP experiment of K-562 using 83803-2-RR

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MITF (83803-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IF Staining of HepG2 using 83803-2-RR

    IF Staining of HepG2 using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 83803-2-RR

    IF Staining of A431 using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83803-2-RR

    IF Staining of HeLa using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83803-2-RR

    FC experiment of HeLa using 83803-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant antibody (83803-2-RR, Clone:240815E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83803-2-RR

    Affinity and Kinetic Characterization of 83803-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83803-2-RR against Human MITF were performed. The affinity constant is 31.9 pM.

MITF Recombinant monoclonal antibody

MITF Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 83803-2-RR

产品说明书

CloneNo. 240815E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

240815E7, bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells, A431 cells
Positive FC (Intra) detected inHeLa cells
Positive ChIP-qPCR detected inK-562 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83803-2-RR targets MITF in IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

CatNo: Ag3679

Product name: Recombinant human MITF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC012503

Sequence: MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEFMKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microphthalmia-associated transcription factor
别名 240815E7, bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor
计算分子量 91 aa, 10 kDa, 59 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC012503
基因名称 MITF
Gene ID (NCBI) 4286
RRIDAB_3671391
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75030
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).

实验方案

Product Specific Protocols
FC protocol for MITF antibody 83803-2-RRDownload protocol
IF protocol for MITF antibody 83803-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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