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验证数据展示

  • WB analysis using CL488-67475

    WB analysis using CL488-67475

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67475 (MIS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67475

    FC experiment of HeLa using CL488-67475

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MIS12 (CL488-67475, Clone:2G5F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody

MIS12 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67475

产品说明书

CloneNo. 2G5F6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra)

别名

Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MIS12 Ready-To-Use IHC Kit. MIS12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67475-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67475 targets MIS12 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag16826

Product name: Recombinant human MIS12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC000229

Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 MIS12, MIND kinetochore complex component, homolog (yeast)
别名 Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6
计算分子量 205 aa, 24 kDa
观测分子量 25 kDa
GenBank蛋白编号BC000229
基因名称 MIS12
Gene ID (NCBI) 79003
RRIDAB_2934544
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H081
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
WB protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
Standard Protocols
Click here to view our Standard Protocols
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