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验证数据展示

  • WB analysis using 83199-2-RR

    WB analysis using 83199-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83199-2-RR (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of Neuro-2a using 83199-2-RR

    FC experiment of Neuro-2a using 83199-2-RR

    1x10^6 Neuro-2a cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83199-2-RR

    Affinity and Kinetic Characterization of 83199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83199-2-RR against Mouse MIF were performed. The affinity constant is 0.131 nM.

MIF Recombinant monoclonal antibody

MIF Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83199-2-RR

产品说明书

CloneNo. 230529F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

230529F2, GIF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, THP-1 cells, RAW 264.7 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inNeuro-2a cells, RAW 264.7 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83199-2-RR targets MIF in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg0574

Product name: Recombinant mouse MIF protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 2-115 aa of NM_010798.2

Sequence: PMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 macrophage migration inhibitory factor
别名 230529F2, GIF
计算分子量13KD
观测分子量13 kDa
GenBank蛋白编号NM_010798.2
基因名称 Mif
Gene ID (NCBI) 17319
RRIDAB_3670887
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP34884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It is involved in various functions, including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumorigenesis, and counter-regulation of glucocorticoids (GC). MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

实验方案

Product Specific Protocols
FC protocol for MIF antibody 83199-2-RRDownload protocol
WB protocol for MIF antibody 83199-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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