• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 83199-2-RR (same clone as 83199-2-PBS)

    WB analysis using 83199-2-RR (same clone as 83199-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83199-2-RR (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • FC experiment of Neuro-2a using 83199-2-RR (same clone as 83199-2-PBS)

    FC experiment of Neuro-2a using 83199-2-RR (same clone as 83199-2-PBS)

    1x10^6 Neuro-2a cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83199-2-PBS

    Affinity and Kinetic Characterization of 83199-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83199-2-PBS against Mouse MIF was performed. The affinity constant is 0.473 nM.

  • Affinity and Kinetic Characterization of 83199-2-RR

    Affinity and Kinetic Characterization of 83199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83199-2-RR against Mouse MIF were performed. The affinity constant is 0.131 nM.

Mouse MIF Recombinant monoclonal antibody, PBS Only

MIF Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83199-2-PBS

产品说明书

CloneNo. 230529F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

230529F2, GIF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

83199-2-PBS targets MIF in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg0574

Product name: Recombinant mouse MIF protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 2-115 aa of NM_010798.2

Sequence: PMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 macrophage migration inhibitory factor
别名 230529F2, GIF
计算分子量13KD
观测分子量13 kDa
GenBank蛋白编号NM_010798.2
基因名称 Mif
Gene ID (NCBI) 17319
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP34884
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It is involved in various functions, including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumorigenesis, and counter-regulation of glucocorticoids (GC). MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

||
New chat Able
正在加载，请稍候...