验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, BT-474 cells, MDA-MB-231 cells, DU 145 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87911-1-RR targets MIEN1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5748 Product name: Recombinant Human MIEN1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 2-115 aa of NM_032339.5 Sequence: SGEPGQTSVAPPPEEVEPGSGVRIVVEYCEPCGFEATYLELASAVKEQYPGIEIESRLGGTGAFEIEINGQLVFSKLENGGFPYEKDLIEAIRRASNGETLEKITNSRPPCVIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chromosome 17 open reading frame 37 |
| 别名 | C17orf37, C35, HBV XAg-transactivated protein 4, HBV X-transactivated gene 4 protein, Migration and invasion enhancer 1 |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | NM_032339.5 |
| 基因名称 | C17orf37 |
| Gene ID (NCBI) | 84299 |
| RRID | AB_3745822 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BRT3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MIEN1 (Migration and Invasion Enhancer 1), also known as C17orf37 or RDEP, is a protein-coding gene located in the q12 region of chromosome 17. Through prenylation modification, this protein anchors to the inner side of the cell membrane, promotes actin cytoskeleton rearrangement and membrane ruffling, thereby enhancing the migration and invasion capabilities of tumor cells. MIEN1 is highly expressed in various malignant tumors, including breast cancer, prostate cancer, and lung cancer, and its expression level is closely correlated with tumor metastasis, clinical stage, and poor patient prognosis. Moreover, MIEN1 promotes epithelial-mesenchymal transition (EMT) by regulating signaling pathways such as PI3K/AKT and NF-κB, making it a potential anti-metastatic therapeutic target and prognostic marker.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIEN1 antibody 87911-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
