验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-13965 targets MICAL2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | MICAL2 fusion protein Ag4950 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | microtubule associated monoxygenase, calponin and LIM domain containing 2 |
别名 | DKFZp686H03148, DKFZp686H2469, KIAA0750, MICAL2, MICAL2PV1, MICAL2PV2, Protein MICAL 2 |
计算分子量 | 127 kDa |
观测分子量 | 95 kDa, 112 kDa |
GenBank蛋白编号 | BC044577 |
基因名称 | MICAL2 |
Gene ID (NCBI) | 9645 |
RRID | AB_2919082 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O94851 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain and a Lin11, Isl-1 and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variants of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 MICAL2 antibody CL488-13965 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |