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验证数据展示

  • FC experiment of HeLa using Biotin-65161

    FC experiment of HeLa using Biotin-65161

    1x10^6 HeLa cells were surface stained with 0.5 ug Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161, Clone:6D4) or isotype control, and SA-PE. Cells were not fixed.

  • IHC staining of human tonsillitis using Biotin-65161

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using Biotin-65161

    IHC staining of human tonsillitis using Biotin-65161

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biotin Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for IHC, FC
Cat No. Biotin-65161

产品说明书

CloneNo. 6D4

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

human

应用

IHC, FC

别名

MICA, MIC-A, MIC A, 6D4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65161 targets MICA/MICB in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原 Transfected C1R cells expressing MICA 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 MHC class I polypeptide-related sequence A
别名 MICA, MIC-A, MIC A, 6D4
计算分子量 383 aa, 43 kDa
GenBank蛋白编号BC016929
基因名称 MICA
Gene ID (NCBI) 100507436
RRIDAB_2883067
偶联类型 Biotin
形式 Liquid
纯化方式The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
UNIPROT IDQ29983
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

实验方案

Product Specific Protocols
IHC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
FC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
Standard Protocols
Click here to view our Standard Protocols