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验证数据展示

  • WB analysis using 86403-3-RR (same clone as 86403-3-PBS)

    WB analysis using 86403-3-RR (same clone as 86403-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86403-3-RR (MIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86403-3-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 86403-3-RR (same clone as 86403-3-PBS)

    IF Staining of A549 using 86403-3-RR (same clone as 86403-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIA3 antibody (86403-3-RR, Clone: 250698A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86403-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86403-3-RR

    Affinity and Kinetic Characterization of 86403-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86403-3-RR against Human MIA3 were performed. The affinity constant is below 1 pM.

MIA3 Recombinant monoclonal antibody, PBS Only

MIA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86403-3-PBS

产品说明书

CloneNo. 250698A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ARNT, C219 reactive peptide, C219-reactive peptide, D320, KIAA0268

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86403-3-PBS targets MIA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag39740

Product name: Recombinant human MIA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 118-467 aa of BC047116

Sequence: KAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRCPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGEPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma inhibitory activity family, member 3
别名 ARNT, C219 reactive peptide, C219-reactive peptide, D320, KIAA0268
计算分子量214 kDa
观测分子量 300 kDa
GenBank蛋白编号BC047116
基因名称 MIA3
Gene ID (NCBI) 375056
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5JRA6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.

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