验证数据展示
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | BALB/c mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 1 publications below |
产品信息
CL647-65122 targets MHC Class II (I-A/I-E) in IF/ICC, FC applications and shows reactivity with Mouse samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 文献引用应用 | IF, FC |
| 经测试反应性 | Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Activated C57BL/6 mouse spleen cells 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histocompatibility 2, class II antigen A, alpha |
| 别名 | Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II |
| GenBank蛋白编号 | BC031711 |
| 基因名称 | MHC Class II |
| Gene ID (NCBI) | 14960 |
| RRID | AB_2883695 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P14434 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 MHC Class II (I-A/I-E) antibody CL647-65122 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Vet Res Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis | ||
Pharmaceuticals (Basel) Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |