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CoraLite® Plus 488-conjugated MGMT Monoclonal antibody

MGMT Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H2C9

Cat No : CL488-67476

Print datasheet

Synonyms

Homo sapiens (Human), MGMT

验证数据展示

  • IF Staining of HepG2 using CL488-67476

    IF Staining of HepG2 using CL488-67476

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated MGMT antibody (CL488-67476, Clone: 1H2C9 ) at dilution of 1:100.

  • FC experiment of HeLa using CL488-67476

    FC experiment of HeLa using CL488-67476

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MGMT (CL488-67476, Clone:1H2C9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy MGMT Ready-To-Use IHC Kit. MGMT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67476-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67476 targets MGMT in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen MGMT fusion protein Ag29936 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name O-6-methylguanine-DNA methyltransferase
Synonyms Homo sapiens (Human), MGMT
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000824
Gene Symbol MGMT
Gene ID (NCBI) 4255
RRIDAB_2919482
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16455
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MGMT antibody CL488-67476Download protocol
FC protocol for CL Plus 488 MGMT antibody CL488-67476Download protocol
Standard Protocols
Click here to view our Standard Protocols