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验证数据展示

  • WB analysis using 86115-5-RR

    WB analysis using 86115-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 89933-4-RR (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 86115-5-RR

    IHC staining of human lung cancer using 86115-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MGMT antibody included
  • IHC staining of human lung cancer using 86115-5-RR

    IHC staining of human lung cancer using 86115-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MGMT antibody included
  • IF Staining of MCF-7 using 86115-5-RR

    IF Staining of MCF-7 using 86115-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGMT antibody (89933-4-RR, Clone: 243130G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MGMT Recombinant antibody

MGMT Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86115-5-RR

产品说明书

CloneNo. 243130G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, Raji cells, HeLa cells, HepG2 cells, LNCaP cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MGMT Ready-To-Use IHC Kit. MGMT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86115-5-RR targets MGMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MGMT fusion protein Ag9996 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 O-6-methylguanine-DNA methyltransferase
别名 6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000824
基因名称 MGMT
Gene ID (NCBI) 4255
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16455
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment (PMID:23065697). MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine (PMID: 17482892). The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea (PMID: 10767620).

实验方案

Product Specific Protocols
WB protocol for MGMT antibody 86115-5-RRDownload protocol
IHC protocol for MGMT antibody 86115-5-RRDownload protocol
IF protocol for MGMT antibody 86115-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols