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验证数据展示

  • WB analysis using 86115-5-RR (same clone as 86115-5-PBS)

    WB analysis using 86115-5-RR (same clone as 86115-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 89933-4-RR (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

    IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MGMT antibody included
  • IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

    IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MGMT antibody included
  • IF Staining of MCF-7 using 86115-5-RR (same clone as 86115-5-PBS)

    IF Staining of MCF-7 using 86115-5-RR (same clone as 86115-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGMT antibody (89933-4-RR, Clone: 243130G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

MGMT Recombinant monoclonal antibody, PBS Only

MGMT Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86115-5-PBS

产品说明书

CloneNo. 243130G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MGMT Ready-To-Use IHC Kit. MGMT primary antibody included.

产品信息

86115-5-PBS targets MGMT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9996

Product name: Recombinant human MGMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-207 aa of BC000824

Sequence: MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 O-6-methylguanine-DNA methyltransferase
别名 6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000824
基因名称 MGMT
Gene ID (NCBI) 4255
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16455
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment (PMID:23065697). MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine (PMID: 17482892). The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea (PMID: 10767620).

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