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验证数据展示

  • WB analysis using 29885-1-AP (same clone as 29885-1-PBS)

    WB analysis using 29885-1-AP (same clone as 29885-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

    IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

    IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

ACTL9 Polyclonal antibody, PBS Only

ACTL9 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29885-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29885-1-PBS targets ACTL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31805

Product name: Recombinant human MGC33407 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-172 aa of BC024028

Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypothetical protein MGC33407
别名 Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407
观测分子量45 kDa
GenBank蛋白编号BC024028
基因名称 ACTL9
Gene ID (NCBI) 284382
RRIDAB_2923617
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC94
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.

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