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验证数据展示

  • WB analysis using 29885-1-AP

    WB analysis using 29885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 29885-1-AP

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 29885-1-AP

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 29885-1-AP

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 29885-1-AP

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 29885-1-AP

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 29885-1-AP

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTL9 Polyclonal antibody

ACTL9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29885-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29885-1-AP targets ACTL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag31805

Product name: Recombinant human MGC33407 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-172 aa of BC024028

Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypothetical protein MGC33407
别名 Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407
观测分子量45 kDa
GenBank蛋白编号BC024028
基因名称 ACTL9
Gene ID (NCBI) 284382
RRIDAB_2923617
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.

实验方案

Product Specific Protocols
IF protocol for ACTL9 antibody 29885-1-APDownload protocol
IHC protocol for ACTL9 antibody 29885-1-APDownload protocol
WB protocol for ACTL9 antibody 29885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

Authors - Chuan Wang
View Article
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