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验证数据展示

  • WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    pig/rat/mouse brain tissues were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFF antibody (66527-1-Ig, Clone: 1G4B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

MFF Monoclonal antibody, PBS Only

MFF Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66527-1-PBS

产品说明书

CloneNo. 1G4B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

1G4B12, AD033, C2orf33, GL004, Mitochondrial fission factor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66527-1-PBS targets MFF in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag9990

Product name: Recombinant human MFF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-238 aa of BC000797

Sequence: MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial fission factor
别名 1G4B12, AD033, C2orf33, GL004, Mitochondrial fission factor
计算分子量 38 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC000797
基因名称 MFF
Gene ID (NCBI) 56947
RRIDAB_2881890
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial Fission Factor (MFF) is a critical regulator of organelle division, specifically playing a central role in the fission of mitochondria and peroxisomes. DRP1, MiD, and MFF form a trimeric complex in which MiD proteins facilitate the MFF-DRP1 interaction. Moreover, MiD51 can inhibit MFF-induced activation of DRP1 GTPase activity. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.(PMID: 39365854, PMID: 37291333)

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