验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66527-1-Ig targets MFF in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, pig, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MFF fusion protein Ag9990 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial fission factor |
| 别名 | AD033, C2orf33, MFF, mitochondrial fission factor |
| 计算分子量 | 38 kDa |
| 观测分子量 | 35-38 kDa |
| GenBank蛋白编号 | BC000797 |
| 基因名称 | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_2881890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9GZY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MFF antibody 66527-1-Ig | Download protocol |
| IHC protocol for MFF antibody 66527-1-Ig | Download protocol |
| IF protocol for MFF antibody 66527-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytother Res Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow | ||
J Adv Res Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics | ||
iScience Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis | ||
Theriogenology OPA1 deficiency induces mitophagy through PINK1/Parkin pathway during bovine oocytes maturation | ||
Respir Res Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia |
