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验证数据展示

  • WB analysis using 33188-1-AP

    WB analysis using 33188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33188-1-AP (MFAP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 33188-1-AP

    WB analysis using 33188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33188-1-AP (MFAP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

MFAP3L Polyclonal antibody

MFAP3L Polyclonal Antibody for WB, ELISA
Cat No. 33188-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

HSD39, HSD-39, KIAA0626, Microfibrillar-associated protein 3-like, Testis development protein NYD-SP9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33188-1-AP targets MFAP3L in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37478

Product name: Recombinant human MFAP3L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-149 aa of NM_021647.7

Sequence: KSVTNSTLNGTNVVLGSVPVIIARTDHIIVKEGNSALINCSVYGIPDPQFKWYNSIGKLLKEEEDEKERGGGKWQMHDSGLLNITKVSFSDRGKYTCVASNIYGTVNNTVTLRVIFTSGDM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microfibrillar-associated protein 3-like
别名 HSD39, HSD-39, KIAA0626, Microfibrillar-associated protein 3-like, Testis development protein NYD-SP9
计算分子量45 kDa，409aa
观测分子量50 kDa
GenBank蛋白编号NM_021647.7
基因名称 MFAP3L
Gene ID (NCBI) 9848
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microfibrillar-associated protein 3-like (MFAP3L) is a member of the microfibril-associated glycoprotein (MAGP) family. MFAP3L exhibits strong expression in the testis. It functions as a dual-specificity kinase, phosphorylating tyrosine and threonine on target proteins. MFAP3L is involved in the nuclear signaling of EGFR and ERK2, playing a role in cell invasion and metastasis in colorectal cancer (PMID: 24735981). It may also participate in the regulation of tumor microenvironment status and molecular subtypes in bladder cancer (PMID: 39489826).

实验方案

Product Specific Protocols
WB protocol for MFAP3L antibody 33188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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