验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22882-1-AP targets MEX3C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MEX3C fusion protein Ag18931 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mex-3 homolog C (C. elegans) |
| 别名 | RING finger protein 194, RING finger and KH domain-containing protein 2, MEX 3C, EC:2.3.2.27, BM-013 |
| 计算分子量 | 659 aa, 69 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC148855 |
| 基因名称 | MEX3C |
| Gene ID (NCBI) | 51320 |
| RRID | AB_2879183 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5U5Q3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEX3C(RNA-binding E3 ubiquitin-protein ligase MEX3C) is also named as RKHD2, RNF194 and may be involved in post-transcriptional regulatory mechanisms. MEX3C is necessary for normal postnatal growth and enhances the expression of local bone Igf1 expression(PMID:22927639). This protein is expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. At least three MEX3C protein variants can be expressed by alternative splicing and alternative transcription initiation: MEX3C659AA(containing 659 amino acid residues, about 82 kDa), MEX3C464AA(about 64 kDa) and MEX3C372AA(about 50 kDa)(PMID: 28982131,27053362,22863774).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEX3C antibody 22882-1-AP | Download protocol |
| IHC protocol for MEX3C antibody 22882-1-AP | Download protocol |
| IF protocol for MEX3C antibody 22882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice. | ||
Biol Reprod Oocyte-Specific Expression of Mouse MEX3C652AA in the Ovary and Its Potential Role in Regulating Maternal Fos mRNA. |
