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验证数据展示

  • WB analysis using 31173-1-AP

    WB analysis using 31173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31173-1-AP (METTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31173-1-AP

    IF Staining of A431 using 31173-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL8 antibody (31173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

METTL8 Polyclonal antibody

METTL8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31173-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

methyltransferase like 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 205 cells, HCT 116 cells, LoVo cells, SW480 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31173-1-AP targets METTL8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 METTL8 fusion protein Ag34222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 8
别名 methyltransferase like 8
计算分子量33 kDa
观测分子量33~40 kDa
GenBank蛋白编号BC025250
基因名称 METTL8
Gene ID (NCBI) 79828
RRIDAB_3669881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL8 (methyltransferase-like protein 8) is an m3C methyltransferase that is best known for its mitochondrial role in installing m3C32 on mt-tRNAThr/Ser(UCN). METTL8 has proved to be a transcriptional target of STAT3 that mediates STAT3's functions in mESCs (PMID: 29706498). METTL8 has multiple splicing isoforms and can undergo sumoylation and associate with nuclear RNA-binding proteins to regulate R-loop formation on ribosomal DNA gene (presumably via its methyltransferase activity on m3C) (PMID: 38744809).

实验方案

Product Specific Protocols
WB protocol for METTL8 antibody 31173-1-APDownload protocol
IF protocol for METTL8 antibody 31173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols