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验证数据展示

  • WB analysis using 17092-1-AP

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17092-1-AP

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL7A antibody included
  • WB analysis of HepG2 using 17092-1-AP

    WB analysis of HepG2 using 17092-1-AP

    WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

METTL7A Polyclonal antibody

METTL7A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17092-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL7A Ready-To-Use IHC Kit. METTL7A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 METTL7A fusion protein Ag10013 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 7A
别名 AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348
计算分子量 28 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC008180
基因名称 METTL7A
Gene ID (NCBI) 25840
RRIDAB_2878348
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for METTL7A antibody 17092-1-APDownload protocol
IHC protocol for METTL7A antibody 17092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols