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验证数据展示

  • WB analysis using 17092-1-AP

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17092-1-AP

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL7A antibody included
  • WB analysis of HepG2 using 17092-1-AP

    WB analysis of HepG2 using 17092-1-AP

    WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

METTL7A Polyclonal antibody

METTL7A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17092-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL7A Ready-To-Use IHC Kit. METTL7A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10013

Product name: Recombinant human METTL7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC008180

Sequence: MELTIFILRLAIYILTFPLYLLNFLGLWSWICKKWFPYFLVRFTVIYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLIKSIAENRHLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLKLQHIQAPLSWELVRPHIYGYAVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 7A
别名 AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348
计算分子量 28 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC008180
基因名称 METTL7A
Gene ID (NCBI) 25840
RRIDAB_2878348
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for METTL7A antibody 17092-1-APDownload protocol
WB protocol for METTL7A antibody 17092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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