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验证数据展示

  • WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MEST antibody included
  • IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MEST antibody included
  • IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

MEST Monoclonal antibody, PBS Only

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60263-1-PBS

产品说明书

CloneNo. 1A4A1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MEST Ready-To-Use IHC Kit. MEST primary antibody included.

产品信息

60263-1-PBS targets MEST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11929

Product name: Recombinant human MEST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-234 aa of BC011908

Sequence: MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWD

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mesoderm specific transcript homolog (mouse)
别名 1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1
计算分子量 38 kDa
观测分子量 34 kDa
GenBank蛋白编号BC011908
基因名称 MEST
Gene ID (NCBI) 4232
RRIDAB_2881384
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5EB52
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

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