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MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat No : 18804-1-AP

Print datasheet

Synonyms

MEPE, OF45, Osteoblast/osteocyte factor 45

验证数据展示

  • WB analysis of HepG2 using 18804-1-AP

    WB analysis of HepG2 using 18804-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18804-1-AP

    WB analysis of HeLa using 18804-1-AP

    WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells.

经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, A375 cells, HeLa cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18804-1-AP targets MEPE in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix extracellular phosphoglycoprotein
Synonyms MEPE, OF45, Osteoblast/osteocyte factor 45
Calculated Molecular Weight 525 aa, 58 kDa
Observed Molecular Weight 43 kDa, 98 kDa
GenBank Accession NumberBC128158
Gene Symbol MEPE
Gene ID (NCBI) 56955
RRIDAB_2143574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).

实验方案

Product Specific Protocols
WB protocol for MEPE antibody 18804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols