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MEF2A Polyclonal antibody

MEF2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28819-1-AP

Print datasheet

Synonyms

ADCAD1, MEF2, MEF2A, myocyte enhancer factor 2A, RSRFC4, RSRFC9

验证数据展示

  • WB analysis using 28819-1-AP

    WB analysis using 28819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28819-1-AP (MEF2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 28819-1-AP

    IHC staining of rat brain using 28819-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28819-1-AP (MEF2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHUVEC cells, SH-SY5Y cells, THP-1 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28819-1-AP targets MEF2A in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen MEF2A fusion protein Ag30349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myocyte enhancer factor 2A
Synonyms ADCAD1, MEF2, MEF2A, myocyte enhancer factor 2A, RSRFC4, RSRFC9
Calculated Molecular Weight 499 aa, 54 kDa
Observed Molecular Weight50-70kDa
GenBank Accession NumberBC013437
Gene Symbol MEF2A
Gene ID (NCBI) 4205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02078
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEF2A belongs to a family of DNA binding regulatory proteins. Each of these proteins which belong to the myocyte-specific enhancer factor-2 (MEF2) family binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. MEF2A plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. MEF2A also has key roles in cardiac and skeletal muscle development. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. This antibody can detect two bands around 60 kDa to 70 kDa, the larger band may correspond to its post-translational modifications.(PMID: 15483225,16484498, 23486945 )

实验方案

Product Specific Protocols
WB protocol for MEF2A antibody 28819-1-APDownload protocol
IHC protocol for MEF2A antibody 28819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols