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验证数据展示

  • FC experiment of HepG2 using CL488-67839

    FC experiment of HepG2 using CL488-67839

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED4 (CL488-67839, Clone:2E6F4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MED4 Monoclonal antibody

MED4 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67839

产品说明书

CloneNo. 2E6F4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, rat

应用

FC (Intra)

别名

MED4, Mediator of RNA polymerase II transcription subunit 4, TRAP36, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component, 2E6F4, Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, HSPC126

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67839-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67839 targets MED4 in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, rat
免疫原

CatNo: Ag8562

Product name: Recombinant human MED4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-270 aa of BC005189

Sequence: MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDGDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKEDEDDVEIMSTDSSSSSSESD

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 4
别名 MED4, Mediator of RNA polymerase II transcription subunit 4, TRAP36, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component, 2E6F4, Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, HSPC126
计算分子量 270 aa, 30 kDa
观测分子量~36 kDa
GenBank蛋白编号BC005189
基因名称 MED4
Gene ID (NCBI) 29079
RRIDAB_2934573
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPJ6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MED4 antibody CL488-67839Download protocol
Standard Protocols
Click here to view our Standard Protocols