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验证数据展示

  • WB analysis of mouse testis using 16590-1-AP (same clone as 16590-1-PBS)

    WB analysis of mouse testis using 16590-1-AP (same clone as 16590-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16590-1-AP (same clone as 16590-1-PBS)

    IF Staining of HepG2 using 16590-1-AP (same clone as 16590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED31 antibody (16590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

MED31 Polyclonal antibody, PBS Only

MED31 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16590-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16590-1-PBS targets MED31 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9008

Product name: Recombinant human MED31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-131 aa of BC012539

Sequence: MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 31
别名
计算分子量 131 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC012539
基因名称 MED31
Gene ID (NCBI) 51003
RRIDAB_2142447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3C7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

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