MED31 Polyclonal antibody

MED31 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16590-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

SOH1, Mediator of RNA polymerase II transcription subunit 31, hSOH1, CGI-125

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, human brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16590-1-AP targets MED31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 MED31 fusion protein Ag9008 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 31
别名 SOH1, Mediator of RNA polymerase II transcription subunit 31, hSOH1, CGI-125
计算分子量 131 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC012539
基因名称 MED31
Gene ID (NCBI) 51003
RRIDAB_2142447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3C7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

实验方案

Product Specific Protocols
WB protocol for MED31 antibody 16590-1-APDownload protocol
IF protocol for MED31 antibody 16590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dev Biol

The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development.

Authors - Risley Michael D MD
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