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验证数据展示

  • WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ME2 Monoclonal antibody, PBS Only

ME2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67457-1-PBS

产品说明书

CloneNo. 6E6E2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

Malic enzyme 2, ME2, NAD ME

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67457-1-PBS targets ME2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag20741

Product name: Recombinant human ME2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 136-480 aa of BC000147

Sequence: SISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGYRIPIC

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 malic enzyme 2, NAD(+)-dependent, mitochondrial
别名 Malic enzyme 2, ME2, NAD ME
计算分子量 65 kDa
观测分子量 54 kDa, 65 kDa
GenBank蛋白编号BC000147
基因名称 ME2
Gene ID (NCBI) 4200
RRIDAB_2882689
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23368
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.