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验证数据展示

  • IP experiment of Kasumi-1 using 31740-1-AP (same clone as 31740-1-PBS)

    IP experiment of Kasumi-1 using 31740-1-AP (same clone as 31740-1-PBS)

    IP result of anti-MDFIC (IP:31740-1-AP, 4ug; Detection:31740-1-AP 1:500) with Kasumi-1 cells lysate 1320 ug. This data was developed using the same antibody clone with 31740-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 31740-1-AP (same clone as 31740-1-PBS)

    IF Staining of HEK-293 using 31740-1-AP (same clone as 31740-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MDFIC antibody (31740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31740-1-PBS in a different storage buffer formulation.

MDFIC Polyclonal antibody, PBS Only

MDFIC Polyclonal Antibody for IF/ICC, IP, Indirect ELISA
Cat No. 31740-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, Indirect ELISA

别名

HIC, I-mfa domain-containing protein, LMPHM12, MyoD family inhibitor domain-containing protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31740-1-PBS targets MDFIC in IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35437

Product name: Recombinant human MDFIC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of NM_001166345

Sequence: MSGAGEALAPGPVGPQRVAEAGGGQLGSTAQGKCDKDNTEKDITQATNSHFTHGEMQDQSIWGNPSDGELIRTQPQRLPQLQTSAQVPSGEEIGKIKNGHTGLSNGNGIHHGAKHGSADNRKLSAPVSQKMHRKIQSSLSVNSDISKKSKVNAVFSQKTGSSPEDCCVHC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MyoD family inhibitor domain containing
别名 HIC, I-mfa domain-containing protein, LMPHM12, MyoD family inhibitor domain-containing protein
计算分子量26 kDa
观测分子量32 kDa
GenBank蛋白编号NM_001166345
基因名称 MDFIC
Gene ID (NCBI) 29969
RRIDAB_3742468
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P1T7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MDFIC encodes the MyoD family inhibitor domain containing protein, a 246-amino acid protein documented to regulate the activity of transcription factors including the glucocorticoid recep tor (GR), HAND1, and T cell factor/lymphoid enhancer factor (TCF/LEF) family members. The C-terminal region of MDFIC harbors a domain extremely rich in cysteine residues, reported to mediate protein-protein interactions between MDFIC and transcription factor componentry (PMID: 35235341).

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