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Species Reactivity
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Conjugate
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验证数据展示

  • IP experiment of Kasumi-1 using 31740-1-AP

    IP experiment of Kasumi-1 using 31740-1-AP

    IP result of anti-MDFIC (IP:31740-1-AP, 4ug; Detection:31740-1-AP 1:500) with Kasumi-1 cells lysate 1320 ug.

  • IF Staining of HEK-293 using 31740-1-AP

    IF Staining of HEK-293 using 31740-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MDFIC antibody (31740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MDFIC Polyclonal antibody

MDFIC Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 31740-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

HIC, I-mfa domain-containing protein, LMPHM12, MyoD family inhibitor domain-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inKasumi-1 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31740-1-AP targets MDFIC in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 MDFIC fusion protein Ag35437 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MyoD family inhibitor domain containing
别名 HIC, I-mfa domain-containing protein, LMPHM12, MyoD family inhibitor domain-containing protein
计算分子量26 kDa
观测分子量32 kDa
GenBank蛋白编号NM_001166345
基因名称 MDFIC
Gene ID (NCBI) 29969
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P1T7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDFIC encodes the MyoD family inhibitor domain containing protein, a 246-amino acid protein documented to regulate the activity of transcription factors including the glucocorticoid recep tor (GR), HAND1, and T cell factor/lymphoid enhancer factor (TCF/LEF) family members. The C-terminal region of MDFIC harbors a domain extremely rich in cysteine residues, reported to mediate protein-protein interactions between MDFIC and transcription factor componentry (PMID: 35235341).

实验方案

Product Specific Protocols
IF protocol for MDFIC antibody 31740-1-APDownload protocol
IP protocol for MDFIC antibody 31740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols