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验证数据展示

  • WB analysis using 34213-1-AP

    WB analysis using 34213-1-AP

    Rat spleen tissue were subjected to SDS PAGE followed by western blot with 34213-1-AP (MD-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 34213-1-AP

    IHC staining of rat spleen using 34213-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 34213-1-AP (MD-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 34213-1-AP

    IHC staining of rat spleen using 34213-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 34213-1-AP (MD-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MD-1 Polyclonal antibody

MD-1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 34213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Ly86, Ly-86, Lymphocyte antigen 86, Md1, Protein MD-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat spleen tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

34213-1-AP targets MD-1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg6369

Product name: Recombinant mouse MD-1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-162 aa of NM_010745.2

Sequence: DHGSENGWPKHTACNSGGLEVVYQSCDPLQDFGLSIDQCSKQIQSNLNIRFGIILRQDIRKLFLDITLMAKGSSILNYSYPLCEEDQPKFSFCGRRKGEQIYYAGPVNNPGLDVPQGEYQLLLELYNENRATVACANATVTSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 86
别名 Ly86, Ly-86, Lymphocyte antigen 86, Md1, Protein MD-1
计算分子量18 kDa
观测分子量24 kDa
GenBank蛋白编号NM_010745.2
基因名称 Ly86
Gene ID (NCBI) 17084
RRIDAB_3743173
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO88188
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lymphocyte antigen 86 (LY86), also known as myeloid differentiation protein 1 (MD1), encodes a secreted glycoprotein that participates in inflammation, humoral immune response, apoptosis, and signal transduction. LY86 is widely expressed in cardiac tissues and predominantly by B cells and macrophages. (PMID: 38822281)

实验方案

Product Specific Protocols
IHC protocol for MD-1 antibody 34213-1-APDownload protocol
WB protocol for MD-1 antibody 34213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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