验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-FcA98044 targets MCP-1/CCL2 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg31375 Product name: Recombinant Human MCP-1/CCL2 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 24-99 aa of BC009716 Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 2 |
| 别名 | CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2 |
| 计算分子量 | 99 aa, 11 kDa |
| GenBank蛋白编号 | BC009716 |
| 基因名称 | MCP-1/CCL2 |
| Gene ID (NCBI) | 6347 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P13500 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, is a chemokine that regulates monocyte and macrophage migration and infiltration. In addition to monocytes and macrophages, MCP-1 can also serve as a chemoattractant for T cells, B cells, NK cells, dendritic cells, and neutrophils. MCP-1 expression is typically induced by the release of inflammatory cytokines and other stimuli from sites of tissue injury and infection. It in turn promotes the extravasation of immune cells across blood vessel walls and their subsequent infiltration of the infection site. It may also play a role in macrophage polarization as well as the differentiation of monocytes into dendritic cells, MSDCs, and osteoclasts. Increased MCP-1 expression has been linked to inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and multiple sclerosis. (PMID: 19441883, 31921102)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE MCP-1/CCL2 antibody PE-FcA98044 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
