验证数据展示
经过测试的应用
| Positive IF/ICC detected in | THP-1 cells |
| Positive FC detected in | U2OS cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98650-1-RR targets MCOLN2 in IF/ICC, FC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mucolipin 2 |
| 别名 | mucolipin 2, Mucolipin-2, Transient receptor potential channel mucolipin 2, TRP ML2, TRPML2 |
| 计算分子量 | 566 aa, 66 kDa |
| GenBank蛋白编号 | BC104891 |
| 基因名称 | MCOLN2 |
| Gene ID (NCBI) | 255231 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8IZK6 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
MCOLN2 (Mucolipin-2), also known as TRPML2, is a member of the transient receptor potential mucolipin (TRPML) family of gated cation channels. Primarily localized in the membranes of endosomes and lysosomes, it plays critical roles in intracellular trafficking, immune responses, and disease progression
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for MCOLN2 antibody 98650-1-RR | Download protocol |
| IF protocol for MCOLN2 antibody 98650-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
