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CoraLite® Plus 488-conjugated MCM7 Polyclonal antibody

MCM7 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11225

Print datasheet

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

验证数据展示

  • IF Staining of A431 using CL488-11225

    IF Staining of A431 using CL488-11225

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MCM7 antibody (CL488-11225) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-11225

    FC experiment of HepG2 using CL488-11225

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MCM7 (CL488-11225) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11225-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11225 targets MCM7 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen MCM7 fusion protein Ag1730 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name minichromosome maintenance complex component 7
Synonyms CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33993
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM7 antibody CL488-11225Download protocol
FC protocol for CL Plus 488 MCM7 antibody CL488-11225Download protocol
Standard Protocols
Click here to view our Standard Protocols