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验证数据展示

  • WB analysis using 10513-1-AP (same clone as 10513-1-PBS)

    WB analysis using 10513-1-AP (same clone as 10513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

    WB analysis of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

    WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

    IP experiment of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

    IP result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

    IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM2 antibody included
  • IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

    IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM2 antibody included
  • FC experiment of NIH/3T3 using 10513-1-AP (same clone as 10513-1-PBS)

    FC experiment of NIH/3T3 using 10513-1-AP (same clone as 10513-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MCM2 Polyclonal antibody, PBS Only

MCM2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 10513-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

BM28, CCNL1, CDCL1, EC:3.6.4.12, KIAA0030

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

产品信息

10513-1-PBS targets MCM2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0798

Product name: Recombinant human MCM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 657-904 aa of BC007670

Sequence: FDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 2
别名 BM28, CCNL1, CDCL1, EC:3.6.4.12, KIAA0030
计算分子量 102 kDa
观测分子量 116-125 kDa
GenBank蛋白编号BC007670
基因名称 MCM2
Gene ID (NCBI) 4171
RRIDAB_2142131
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49736
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

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