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验证数据展示

  • IHC staining of mouse heart using 11656-2-AP

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of SH-SY5Y using 11656-2-AP

    WB analysis of SH-SY5Y using 11656-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11656-2-AP (MCHR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11656-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11656-2-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2224

Product name: Recombinant human MCHR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-422 aa of BC021146

Sequence: MSVGAMKKGVGRAVGLGGGSGCQATEEDPLPDCGACAPGQGGRRWRLPQPAWVEGSSARLWEQATGTGWMDLEASLLPTGPNASNTSDGPDNLTSAGSPPRTGSISYINIIMPSVFGTICLLGIIGNSTVIFAVVKKSKLHWCNNVPDIFIINLSVVDLLFLLGMPFMIHQLMGNGVWHFGETMCTLITAMDANSQFTSTYILTAMAIDRYLATVHPISSTKFRKPSVATLVICLLWALSFISITPVWLYARLIPFPGGAVGCGIRLPNPDTDLYWFTLYQFFLAFALPFVVITAAYVRILQRMTSSVAPASQRSIRLRTKRVTRTAIAICLVFFVCWAPYYVLQLTQLSISRPTLTFVYLYNAAISLGYANSCLNPFVYIVLCETFRKRLVLSVKPAAQGQLRAVSNAQTADEERTESKGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanin-concentrating hormone receptor 1
别名 MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24
计算分子量 422 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC021146
基因名称 MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143940
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99705
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MCHR1 antibody 11656-2-APDownload protocol
WB protocol for MCHR1 antibody 11656-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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