验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
24610-1-AP targets MBNL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20113 Product name: Recombinant human MBNL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 156-230 aa of BC042090 Sequence: SAASAMALQPGTLQLIPKRSALEKPNGATPVFNPTVFHCQQALTNLQLPQPAFIPAGPILCMAPASNIVPMMHGA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | muscleblind-like 3 (Drosophila) |
| 别名 | CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, MBNL3, MBXL, Muscleblind like protein 3, Protein HCHCR |
| 计算分子量 | 354 aa, 39 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC042090 |
| 基因名称 | MBNL3 |
| Gene ID (NCBI) | 55796 |
| RRID | AB_2879637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NUK0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MBNL3, also named as Muscleblind-like protein 3 or Cys3His CCG1-required protein, is a 354 amino acid protein, which contains four C3H1-type zinc fingers and belongs to the muscleblind family. MBNL3 localizes in the nucleus and is highly expressed in the placenta. MBNL3 mediates pre-mRNA alternative splicing regulation and plays a role in myotonic dystrophy pathophysiology. MBNL3 could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MBNL3 antibody 24610-1-AP | Download protocol |
| WB protocol for MBNL3 antibody 24610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep MBNL3 regulates tumor progression and metastasis in cholangiocarcinoma and serves as a prognostic biomarker. | ||
Cell Cycle TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs. |
