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验证数据展示

  • WB analysis using 85835-4-RR

    WB analysis using 85835-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85835-4-RR (Mbl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 85835-4-RR

    WB analysis of rat liver using 85835-4-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85835-4-RR (Mbl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MBL Recombinant monoclonal antibody

MBL Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85835-4-RR

产品说明书

CloneNo. 250108E11

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

Mannan-binding protein, Mannose-binding protein C, Mbl2, MBP-C, RA-reactive factor P28A subunit

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, mouse serum

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85835-4-RR targets MBL in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3157

Product name: Recombinant Mouse Mbl2 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-244 aa of NM_010776.1

Sequence: ETLTEGVQNSCPVVTCSSPGLNGFPGKDGRDGAKGEKGEPGQGLRGLQGPPGKVGPTGPPGNPGLKGAVGPKGDRGDRAEFDTSEIDSEIAALRSELRALRNWVLFSLSEKVGKKYFVSSVKKMSLDRVKALCSEFQGSVATPRNAEENSAIQKVAKDIAYLGITDVRVEGSFEDLTGNRVRYTNWNDGEPNNTGDGEDCVVILGNGKWNDVPCSDSFLAICEFSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mannose-binding lectin (protein C) 2
别名 Mannan-binding protein, Mannose-binding protein C, Mbl2, MBP-C, RA-reactive factor P28A subunit
计算分子量26 kDa
观测分子量 32 kDa
GenBank蛋白编号NM_010776.1
基因名称 Mbl2
Gene ID (NCBI) 17195
RRIDAB_3744411
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP41317
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mannan-binding lectin (MBL) is a calcium-dependent (type C) serum lectin protein that is synthesized in the liver and circulates in the blood. It plays the role of a pattern recognition receptor in the innate immune system. It can recognize the carbohydrate structures on the surface of microorganisms and activate the lectin complement pathway through serine proteases related to mannan-binding lectins (MASPs), thereby achieving phagocytosis and inflammatory responses without the involvement of antibodies.(PMID: 16948640; 16395391)

实验方案

Product Specific Protocols
WB protocol for MBL antibody 85835-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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