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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MBIP Monoclonal antibody

MBIP Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6H11

Cat No : CL488-66102

Print datasheet

Synonyms

MBIP, MUK binding inhibitory protein

验证数据展示

  • FC experiment of HeLa using CL488-66102

    FC experiment of HeLa using CL488-66102

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MBIP (CL488-66102, Clone:2E6H11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66102-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-66102 is MBIP Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen MBIP fusion protein Ag18090 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MAP3K12 binding inhibitory protein 1
Synonyms MBIP, MUK binding inhibitory protein
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC005197
Gene Symbol MBIP
Gene ID (NCBI) 51562
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS73
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MBIP antibody CL488-66102Download protocol
Standard Protocols
Click here to view our Standard Protocols