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验证数据展示

  • IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

    IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

    IHC staining of human prostate cancer using 82852-3-RR (same clone as 82852-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82852-3-RR (same clone as 82852-3-PBS)

    IF Staining of U2OS using 82852-3-RR (same clone as 82852-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:950 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82852-3-RR (same clone as 82852-3-PBS)

    IF Staining of HeLa using 82852-3-RR (same clone as 82852-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82852-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82852-3-RR

    Affinity and Kinetic Characterization of 82852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82852-3-RR against Human MAZ were performed. The affinity constant is below 1 pM.

MAZ Recombinant monoclonal antibody, PBS Only

MAZ Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 82852-3-PBS

产品说明书

CloneNo. 1D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

1D5, MAZI, Myc-associated zinc finger protein, Pur 1, PUR1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82852-3-PBS targets MAZ in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15200

Product name: Recombinant human MAZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC041629

Sequence: MVPLSLLSVPQLSGAGGGGGEAGAGGGAAAVAAGGVVTTTASGKRIRKNHACEMCGKAFRDVYHLNRHKLSHSDEKPYQCPVCQQRFKRKDRMSYHVRSHDGAVHKPYNCSHCGKSFSRPDHLNSHVRQVHSTERPFKCEKCEAAFATKDRLRAHTVRHEEKVPCHVCGKMLSSAYISDHMKVHSQGPHHVCELCNKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MYC-associated zinc finger protein (purine-binding transcription factor)
别名 1D5, MAZI, Myc-associated zinc finger protein, Pur 1, PUR1
计算分子量 477 aa, 49 kDa
观测分子量 49-51 kDa
GenBank蛋白编号BC041629
基因名称 MAZ
Gene ID (NCBI) 4150
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56270
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).

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