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验证数据展示

  • ChIP experiment of NCCIT using 82958-1-RR

    ChIP experiment of NCCIT using 82958-1-RR

    Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MAX (82958-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 82958-1-RR

    WB analysis using 82958-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82958-1-RR

    WB analysis of HEK-293 using 82958-1-RR

    WB result of MAX antibody (82958-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAX transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82958-1-RR

    Affinity and Kinetic Characterization of 82958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-1-RR against Human MAX were performed. The affinity constant is 0.257 nM.

MAX Recombinant monoclonal antibody

MAX Recombinant Antibody for WB, ELISA
Cat No. 82958-1-RR

产品说明书

CloneNo. 230215E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy MAX Ready-To-Use IHC Kit. MAX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82958-1-RR targets MAX in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0680

Product name: Recombinant human MAX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC003525

Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MYC associated factor X
别名 bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4
计算分子量 18 kDa
观测分子量22 kDa
GenBank蛋白编号BC003525
基因名称 MAX
Gene ID (NCBI) 4149
RRIDAB_3670702
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61244
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

实验方案

Product Specific Protocols
WB protocol for MAX antibody 82958-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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