• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MATCHF1 Polyclonal antibody

MATCHF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 24771-1-AP

Print datasheet

Synonyms

MARCH I, MARCH1, MARCHF1, RING finger protein 171, RNF171

验证数据展示

  • WB analysis using 24771-1-AP

    WB analysis using 24771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24771-1-AP (MATCHF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24771-1-AP targets MATCHF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MATCHF1 fusion protein Ag20231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane-associated ring finger (C3HC4) 1
Synonyms MARCH I, MARCH1, MARCHF1, RING finger protein 171, RNF171
Calculated Molecular Weight 289 aa, 32 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC153124
Gene Symbol MARCH1
Gene ID (NCBI) 55016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).

实验方案

Product Specific Protocols
WB protocol for MATCHF1 antibody 24771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols