验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung tissue, mouse lung tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MAT2B Ready-To-Use IHC Kit. MAT2B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 22 publications below |
| IHC | See 1 publications below |
产品信息
15952-1-AP targets MAT2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken |
| 免疫原 | MAT2B fusion protein Ag8649 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methionine adenosyltransferase II, beta |
| 别名 | Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Nbla02999, MSTP045, MATIIbeta, MAT II beta |
| 计算分子量 | 36 kDa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC005218 |
| 基因名称 | MAT2B |
| Gene ID (NCBI) | 27430 |
| RRID | AB_10637268 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZL9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAT2B antibody 15952-1-AP | Download protocol |
| IHC protocol for MAT2B antibody 15952-1-AP | Download protocol |
| IF protocol for MAT2B antibody 15952-1-AP | Download protocol |
| IP protocol for MAT2B antibody 15952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. | ||
J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. | ||
Sci Rep In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications. | ||
J Nutr Biochem Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats. |
