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MASP1 Polyclonal antibody

MASP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 21837-1-AP

Print datasheet

Synonyms

Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5

验证数据展示

  • WB analysis of HeLa using 21837-1-AP

    WB analysis of HeLa using 21837-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21837-1-AP (MASP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21837-1-AP

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 21837-1-AP

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21837-1-AP is MASP1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MASP1 fusion protein Ag16432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
Synonyms Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5
Calculated Molecular Weight 728 aa, 82 kDa
Observed Molecular Weight 40-70 kDa
GenBank Accession NumberBC106945
Gene Symbol MASP1
Gene ID (NCBI) 5648
RRIDAB_2878924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48740
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MASP1 antibody 21837-1-APDownload protocol
IHC protocol for MASP1 antibody 21837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation

Authors - Binshan Zhang
View Article
human,mouseWB,IHC,IP

Hepatology

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis

Authors - Xianzhi Liu
  • KD Validated
View Article