验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
25667-1-AP targets MARVELD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag22492 Product name: Recombinant human MARVELD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC013376 Sequence: MEDPSGAREPRARPRERDPGRRPHPDQGRTHDRPRDRPGDPRRKRSSDGNRRRDGDRDPKRDQERDGNRDRNRDRERERERERDPDRGPRRDTHRDAGPRAGEHGVWEKPRQSRTRDGARGLTWDAAAPPGPAPWEAPEPPQPQRKGDPGRRRPESEPPSERYLPSTPRPGREEVEYYQSEAEGLLECH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MARVEL domain containing 3 |
| 别名 | MARVD3, MARVEL domain containing 3, MARVELD3, MRVLDC3 |
| 计算分子量 | 401 aa, 45 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC013376 |
| 基因名称 | MARVELD3 |
| Gene ID (NCBI) | 91862 |
| RRID | AB_2880184 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96A59 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| IHC protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| WB protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers | ||
FEBS Open Bio Hesperidin enhances intestinal barrier function in Caco-2 cell monolayers via AMPK-mediated tight junction-related proteins | ||
Mol Med Upregulation of PRRX2 by silencing Marveld3 as a protective mechanism against radiation-induced ferroptosis in skin cells |
