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MARVELD3 Polyclonal antibody

MARVELD3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25667-1-AP

Print datasheet

Synonyms

MARVD3, MARVEL domain containing 3, MARVELD3, MRVLDC3

验证数据展示

  • WB analysis of COLO 320 using 25667-1-AP

    WB analysis of COLO 320 using 25667-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25667-1-AP (MARVELD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25667-1-AP

    IHC staining of human kidney using 25667-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 25667-1-AP

    IHC staining of human kidney using 25667-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 25667-1-AP

    IF Staining of Caco-2 using 25667-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using MARVELD3 antibody (25667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 25667-1-AP is MARVELD3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MARVELD3 fusion protein Ag22492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MARVEL domain containing 3
Synonyms MARVD3, MARVEL domain containing 3, MARVELD3, MRVLDC3
Calculated Molecular Weight 401 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC013376
Gene Symbol MARVELD3
Gene ID (NCBI) 91862
RRIDAB_2880184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).

实验方案

Product Specific Protocols
WB protocol for MARVELD3 antibody 25667-1-APDownload protocol
IHC protocol for MARVELD3 antibody 25667-1-APDownload protocol
IF protocol for MARVELD3 antibody 25667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers

Authors - Ha-Young Park
View Article
humanWB

FEBS Open Bio

Hesperidin enhances intestinal barrier function in Caco-2 cell monolayers via AMPK-mediated tight junction-related proteins

Authors - Ha-Young Park
View Article