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验证数据展示

  • WB analysis of HeLa using 86188-1-RR

    WB analysis of HeLa using 86188-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86188-1-RR (MARVELD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 86188-1-RR

    IF Staining of MCF-7 using 86188-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MARVELD2 antibody (86188-1-RR, Clone: 250774G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86188-1-RR

    Affinity and Kinetic Characterization of 86188-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86188-1-RR against Human MARVELD2 were performed. The affinity constant is 1.19 nM.

MARVELD2 Recombinant monoclonal antibody

MARVELD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86188-1-RR

产品说明书

CloneNo. 250774G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DFNB49, MARVD2, MARVEL domain containing 2, MARVEL domain-containing protein 2, MRVLDC2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MARVELD2 Ready-To-Use IHC Kit. MARVELD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86188-1-RR targets MARVELD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4446

Product name: Recombinant human MARVELD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 365-558 aa of BC033689

Sequence: LWRHEAARRHREYMEQQEINEPSLSSKRKMCEMATSGDRQRDSEVNFKELRTAKMKPELLSGHIPPGHIPKPIVMPDYVAKYPVIQTDDERERYKAVFQDQFSEYKELSAEVQAVLRKFDELDAVMSRLPHHSESRQEHERISRIHEEFKKKKNDPTFLEKKERCDYLKNKLSHIKQRIQEYDKVMNWDVQGYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MARVEL domain containing 2
别名 DFNB49, MARVD2, MARVEL domain containing 2, MARVEL domain-containing protein 2, MRVLDC2
计算分子量 558 aa, 64 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC033689
基因名称 MARVELD2
Gene ID (NCBI) 153562
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N4S9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.

实验方案

Product Specific Protocols
IF protocol for MARVELD2 antibody 86188-1-RRDownload protocol
WB protocol for MARVELD2 antibody 86188-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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