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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Daudi using 85308-2-RR (same clone as 85308-2-PBS)

    WB analysis of Daudi using 85308-2-RR (same clone as 85308-2-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

  • WB analysis using 85308-2-RR (same clone as 85308-2-PBS)

    WB analysis using 85308-2-RR (same clone as 85308-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85308-2-RR

    Affinity and Kinetic Characterization of 85308-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

MARCH1 Recombinant antibody, PBS Only

MARCH1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85308-2-PBS

产品说明书

CloneNo. 242143G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85308-2-PBS targets MARCH1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MARCH1 fusion protein Ag20231 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane-associated ring finger (C3HC4) 1
别名 MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I
计算分子量 289 aa, 32 kDa
观测分子量31-35 kDa
GenBank蛋白编号BC153124
基因名称 MARCHF1
Gene ID (NCBI) 55016
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TCQ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).