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验证数据展示

  • WB analysis of Daudi using 85308-2-RR

    WB analysis of Daudi using 85308-2-RR

    Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85308-2-RR

    WB analysis using 85308-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85308-2-RR

    IHC staining of mouse brain using 85308-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85308-2-RR (MARCH1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85308-2-RR

    Affinity and Kinetic Characterization of 85308-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

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MARCH1 Recombinant monoclonal antibody

MARCH1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85308-2-RR

产品说明书

CloneNo. 242143G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, Daudi cells, rat brain tissue, human skeletal muscle tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85308-2-RR targets MARCH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20231

Product name: Recombinant human MARCH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 222-272 aa of BC153124

Sequence: QNCPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane-associated ring finger (C3HC4) 1
别名 MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I
计算分子量 289 aa, 32 kDa
观测分子量31-35 kDa
GenBank蛋白编号BC153124
基因名称 MARCHF1
Gene ID (NCBI) 55016
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TCQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).

实验方案

Product Specific Protocols
WB protocol for MARCH1 antibody 85308-2-RRDownload protocol
IHC protocol for MARCH1 antibody 85308-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols