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验证数据展示

  • WB analysis of mouse lung using 13452-1-AP

    WB analysis of mouse lung using 13452-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13452-1-AP (MAPK15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 13452-1-AP

    IP experiment of mouse lung using 13452-1-AP

    IP result of anti-MAPK15 (IP:13452-1-AP, 4ug; Detection:13452-1-AP 1:500) with mouse lung tissue lysate 3800ug.

  • IHC staining of human kidney using 13452-1-AP

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 13452-1-AP

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 40x lens).

MAPK15 Polyclonal antibody

MAPK15 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13452-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13452-1-AP targets MAPK15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MAPK15 fusion protein Ag4255 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 15
别名 ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15
计算分子量 544 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC028034
基因名称 MAPK15
Gene ID (NCBI) 225689
RRIDAB_2877950
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK15 antibody 13452-1-APDownload protocol
IHC protocol for MAPK15 antibody 13452-1-APDownload protocol
IP protocol for MAPK15 antibody 13452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Metastasis-associated gene MAPK15 promotes the migration and invasion of osteosarcoma cells via the c-Jun/MMPs pathway.

Authors - Zexin Su
View Article